Apr 20, 2023

In Vitro Therapy: Essential Gases and Medical Testing Solutions

In vitro therapy, a significant part of assisted reproductive technology (ART), has revolutionized the field of reproductive medicine, offering hope to numerous couples struggling with infertility. This article examines the crucial role that certain gases play in maintaining an ideal environment within the laboratory setting, as well as the medical testing solutions that facilitate these procedures.

In vitro fertilization (IVF) and other forms of ART have emerged as groundbreaking medical techniques, offering promising therapeutic solutions to a variety of infertility cases. An essential component of these procedures is the optimization of laboratory conditions, which includes providing the appropriate gaseous environment. This article discusses the importance of specific gases in in vitro therapy and the relevant medical testing solutions that facilitate their installation and monitoring.

I. Essential Gases in In Vitro Therapy

Carbon Dioxide (CO2)
Carbon dioxide plays a vital role in maintaining the optimal pH for embryo culture media. Incubators that house developing embryos typically require CO2 concentrations between 5% and 6%. This helps to maintain a pH of approximately 7.2 – 7.4, which mimics the environment of the female reproductive tract, thereby promoting embryo viability and development.

Oxygen (O2)
Oxygen is an essential gas for cellular respiration, as it is a vital electron acceptor in the production of adenosine triphosphate (ATP), the energy source for cellular processes. In in vitro therapy, maintaining an oxygen concentration of 5 – 20% is critical for supporting the metabolism of the growing embryo. Lowering O2 concentration can also help to reduce the production of reactive oxygen species (ROS), which can lead to oxidative stress and compromise embryo development.

Nitrogen (N2)
Nitrogen is an inert gas that is often used to displace oxygen in controlled laboratory environments. In the context of in vitro therapy, nitrogen is used to create a hypoxic environment, as it allows for the regulation of O2 concentrations. This can help to mimic the in vivo environment more closely and improve embryo culture conditions.

II. Medical Testing Solutions

Gas Monitoring Systems

It is crucial to maintain accurate and consistent gas levels in the in vitro therapy laboratory. Advanced gas monitoring systems can ensure that gas concentrations remain within the appropriate range for optimal embryo development. These systems can be integrated with alarms to alert staff of any deviations from ideal gas levels, allowing for prompt intervention.

Gas Delivery Systems

Proper gas delivery is essential to maintain the desired gaseous environment. Medical-grade gas cylinders, gas generators, and gas mixing systems can be employed to supply the necessary gases to the laboratory. Medical testing solutions can assist in the installation of these systems, ensuring their safe and effective operation.

Gas Purity and Quality Control

To minimize the risk of contamination and adverse effects on embryo development, it is crucial to use high-quality, medical-grade gases. Medical testing solutions can provide routine gas purity testing and quality control checks, ensuring that gases are free from contaminants such as particulate matter, moisture, and volatile organic compounds (VOCs).

In vitro therapy is a highly specialized and sensitive medical procedure that relies on the appropriate gaseous environment for successful outcomes. Medical testing solutions play a vital role in providing the necessary gases and monitoring their levels, ensuring optimal laboratory conditions for embryo culture and development. By understanding the importance of essential gases and leveraging advanced medical testing solutions, in vitro therapy can continue to provide hope and solutions for couples experiencing infertility.

Frequently Asked Questions

Yes, MTS has various partnerships with a number of medical gas installation contractors. Each sub contractor employees qualifying ASSE 6010 Medical Gas Installers ready to meet your needs.
A Medical Gas Certification (Also called a medical gas verification) is an NFPA required inspection and approval of newly installed medical gas delivery systems. All newly in installed medical gas pipeline and equipment must be certified by an ASSE 6030 before use.
Answer: Per NFPA guidelines, it is a requirement for any facilities using medical grade gases for the purpose of human consumption to have their gas delivery systems inspected by and ASSE 6040. Read more about Medical Gas Inspections Here.
Answer: Yes, MTS provides annual medical gas inspections. We provide discounts on Annual Inspections when you sign multi year contracts. Read more about Medical Gas Inspections Here.
Answer: The Plumbing and Mechanical Systems Board issues medical gas piping certification to medical gas system installers who possess valid certification from the National Inspection Testing Certification Corporation or NITC. Certification from other organizations may be considered with approval of the board.
Yes, we can adapt any fittings to the desired hosing for any medical grade gas. High pressure gases such as Nitrogen can also be accommodated.
Yes, Those items for shipment to countries outside of the U.S. may be subject to taxes, customs duties and fees levied by the destination country (“Import Fees”). The recipient of the shipment is the importer of record in the destination country and is responsible for all Import Fees.
With respect to each item for which Import Fees have been calculated, you authorize Export or Merchant (as applicable) to designate a carrier (“Designated Carrier”) to act as your agent with the relevant customs and tax authorities in the destination country, to clear your merchandise, process and remit your actual Import Fees for such item.
Import Fees Deposit” represents an estimate of the Import Fees that will be levied on the items in your order for shipment to countries outside of the U.S. By placing your order, you agree to allow Export and/or Merchant (as applicable) to collect the Import Fees Deposit for the applicable items in your order. This deposit will be used, on your behalf, to reimburse the Designated Carriers for the import fees that they have paid on your behalf to the appropriate authorities of the destination country.
Answer: Per NFPA guidelines, it is a requirement for any facilities using medical grade gases for the purpose of human consumption to have their gas delivery systems inspected by and ASSE 6040. Read more about Medical Gas Inspections Here.

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MTS is an ASSE 6030© Licensed medical gas verification company and providing NFPA code compliant medical gas certification services all across the U.S.

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MTS is an ASSE 6020© Licensed medical gas inspection service company, providing annual inspections on medical gas systems throughout the U.S.

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MTS provides medical gas systems installations per the NFPA 99  using our certified ASSE 6010© Licensed medical gas plumbers all across the U.S.

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MTS is an ASSE 6040© Licensed medical gas maintenance company providing the highest quality medical gas maintenance services all across the U.S.

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